Composite

Part:BBa_K2922007:Design

Designed by: Tianyu Feng   Group: iGEM19_XMU-China   (2019-10-13)


Expression system of YebF-CenA coding YebF-Endoglucanase A with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 450
    Illegal NotI site found at 1594
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1507
    Illegal BamHI site found at 643
    Illegal XhoI site found at 1005
    Illegal XhoI site found at 1254
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 689


Design Notes

Construct from existed parts according to Standard BioBrick Assembly.


Source

YebF comes from Escherichia coli (Strain K12) and cenA is from Cellulomonas fimi.

References